Best method for extracting DNA&RNA from whole blood?

09 September 2018 1 6K Report

I have small volumes (200uL) of whole blood stored in RNALater, and I need to get both DNA and mRNA out. Can anyone recommend good kits/methods?

Thanks so much!

Sadiq S Mareai

you can refer this attached protocol of mr MKV...

Badges
Science topic

Similar topics
Phytochemistry
Extracts

More David J Martino's questions See All

Can you store cells in RNAlater for ATAC-seq?

Dear crowd, Can haematopoetic cells sorted by flow cytometry safely be stored in RNAlater for ATAC seq? Any risk of compromising the integrity of nuclei? Thanks

03 April 2018 6,824 3 View

How to appropriately design a replication study in genomics?

What are the best ways to design a discovery and replication association study in GWAS or EWAS? Should the total samples available within your funding budget be split into two for discovery and...

01 February 2016 5,365 1 View

Does magnetic separation of T cells using DYNAL beads activate them?

DYNAL beads are a great way to purify T-cells from bulk PBMC cultures. There are positive and negative selection strategies. I've heard it said that positive selection using DYNAL beads can induce...

08 September 2013 243 13 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View