I am exploring potential chemical based approaches for cell cycle synchronization and would like to hear from anyone with experience on this technique what they believe to be the most applicable approach with respect to ease of use, reproducibly, cost, etc. An ideal technique would be one that is easily reversed allowing for dissection of multiple stages in a synchronized fashion..
We work on mammalian circadian biology and synchronize our cells with dexamethasone. Ther are many circadian biology papers where you can cite and pull the protocol from.
Yes, Dexamethasone is very effective and helpful for phase locking and cells synchronisation.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4103330/#:~:text=Dexamethasone%2Dinduced%20synchronization%20reveals%20additional,these%20states%20after%20dexamethasone%20synchronization.
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