Hello,
I'm looking to isolate plasma cells and plasmablasts from primary B-cells obtained from mouse spleen (I plan to use CD138 to enrich for plasma cells and plasmablasts). My goal is to culture these cells and maintain their viability for 1-2 weeks. What type of media and cell activators would you recommend for this? Additionally, can I culture the cells in this media immediately after harvesting them?
This reference is worth looking at.
https://pubmed.ncbi.nlm.nih.gov/30209264/
Thank you, Greg A Kirchenbaum. It seems this paper focuses more on human plasma cells (antibody-secreting cells) and their in vitro survival mechanisms, particularly mimicking the bone marrow microenvironment.
I believe that complete RPMI-1640 (10% FBS, Pen-Strep-Gen, enriched with mercaptoethanol should do the trick. DMEM would be an option as well.
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