Using kits for RNA extraction from seeds ends up with poor yield and quality, most of the times. Qiagen RNAeasy kit had failed to give me any good RNA from Arabidopsis seeds. Since quite a lot of seeds are invested for RNA extraction, I would recommend using trizol-based conventional methods. I have been using a method given in the following link. It's a bit lengthy, but assures good quality RNA with decent yields.

http://onlinelibrary.wiley.com/doi/10.1002/biot.200900211/abstract

Thank you Yadukrishnan. I agree. I always end up with poor quality RNA. I have tried a few different methods and haven't had success yet. I will check out the link you posted. Thanks again.

Keep in mind that Arabidopsis seeds have a very high oil content, which makes it difficult to extract any nucleic acids.

Check out this online protocol:

https://www.jove.com/video/55008/an-efficient-method-for-isolation-highly-purified-rna-from-seeds-for

Thanks Katie this is very helpful.

There is no big difference between RLT and RLC. Try trizol and see what is the result.

RLC is better and used generally to all tissue except those tissue cause solidification during RAM isolation..

during RNA isolation

Thanks for the comments and suggestions