Best internal control for miRNA qPCR study?

More Samarjit Maharana's questions See All

What is Ca5.40Mg0.60C6.00O18.00 (detected in XRD)? Is it same as dolomite?

20 April 2023 6,742 2 View

What is the hydrated radii of Re4+ ion?

While searching, I got the ionic radii of various oxidation states of Re but I am unable to fetch the hydrated ionic radius of Re4+ ion. Help me.

02 April 2023 8,481 0 View

Which SMOTE algorithm should I use for Augmentation of Time Series dataset?

I am working on a Time Series Dataset where i want to do forcasting and prediction both. So, if you have any suggestion please share. Thank You!

20 October 2022 5,342 4 View

How to calculate linear range and LOD from fluorescence sensitivity plot?

I have taken a plot of the sensitivity of my probe towards a metal ion. It has been found that with an increase in the concentration of metal ion, fluorescence intensity keeps on quenching. From...

16 March 2021 429 2 View

What is the importance of analysis of citric acid soluble P(CSP) and citric acid insoluble P (CISP), of a sample and what is the procedure of analysi?

I am working on Organo mineral fertilizer and I need to do the Citric acid soluble P (CSP)and Citric acid insoluble P (CISP). So I nned the protocol and also wants to know what is the importance...

14 October 2020 7,180 1 View

What should be the range of ideal retention time in the reactor of the product gas in a fluized bed gasification in labotory scale ??

Designing a fluidsied bed reactor in labotory scale..the dia of bed is 8 cm..

24 February 2020 4,577 2 View

Can we compare two different flow cytometer results of same type of experiments ?

Hello Dear all, I am using BD Canto-II for my experiments. But, due to some technical issue I have shifted to BD Canto-I. So, I have some of my test results from CANTO-II and some of the result...

28 July 2019 6,181 7 View

Is there any solid evidence on leaching activities of POPs /or PHCs associated with plastic fragments after ingestion by marine biota?

Some additives used in the plastic materials are surfactants but frequently they are not used for their surface or interfacial activity. Surfactant-like stuffs may increase the leaching...

03 January 2018 7,511 2 View

Does the material (ultrahigh molecular weight polyethylene) really causing ecotoxicity?

As we know that ultrahigh molecular weight polyethylene (UHMWPE) has extremely low coefficient of friction and it is highly resistant to foreign chemicals.

14 December 2017 1,448 4 View

SEM and EDAX of low grade Manganese ore?

I am confused regarding the conductivity of a low-grade Manganese sample. It consists of more than Manganese mineral alongwith haematite,Goethite,Clay etc.I want to SEM and EDAX of the sample.For...

05 November 2017 3,033 3 View

Are there any good simple systems or platforms to recommend?

In order to show people the beauty of control and enhance enthusiasm for learning control theories, are there any good simple systems or platforms to recommend?

05 August 2024 10,034 1 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,289 1 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

Are current regulations effective in preventing cancer caused by toxins?

I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from experts in the field. If you are an expert or researcher working on...

02 August 2024 4,474 2 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

Some new emerging problems on application of RL for scheduling in IoT networks?

I have seen plenty of existing works on applied Reinforcement Learning (RL) policies for optimized scheduling in IoT networks including Q-learning, DQNs, and the newer ones including PPO for...

01 August 2024 8,754 2 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

How to quantify the fluorescence intensity of only the merge area of an image using ImageJ software?

Dear All: I have to quantify the fluorescence intensity of cells that are fluorescent to two different markers, so i'm interested in finding out if this is possible. Previously I have...

22 July 2024 8,770 1 View

Vipul Batra

The microRNA (cel-miR-39) is a well-accepted microRNA sequence used for normalization in gene expression studies which is compatible to expression analysis using RT-qPCR - both RNA and matching forward PCR primer provided.

Samarjit Maharana

Thanks, Dr. Batra, It would be kind of help if you can share the paper link.