How to prevent MBP protein associate with protein of interest after TEV protease cleavage?

Hi All, I have used MBP tag to help solubilize my target protein. It works really well. However, whenever I cleave off the His-MBP tag and try to clean up the sample by passing through another Ni...

02 March 2019 894 6 View

Does anyone has good DNA degradase protocol (DNA to nucleoside)?

Hi All, I am trying to establish a good DNA degradase protocol for characterizing the global DNA methylation level of mammalian cell genomic DNA via LC-MS. I uses the DNA degradase plus from...

05 June 2018 2,829 12 View

Cheap & good stereo zoom microscope for manipulating protein crystals?

Hi All, Could anyone suggest a economic & good stereo zoom microscope that can allow me to inspect & manipulate protein crystals (with the cryoloops)? We don't need polarized light. As...

02 March 2018 3,019 1 View

How did HeLa cells contaminated so many cell lines?

Hi All, I am just curious about how HeLa cell contaminated so many cell lines? I mean, if we use disposable pipettes, disposable aspirating pipettes, disposable tips, HeLa cell shouldn't be...

01 February 2018 529 5 View

Can anyone suggest cancer cell lines that are highly sensitive to p53 overexpression?

Hi All, I want to select a cell line for our drug study but it has to be highly sensitive to p53 induction - i.e. overexpression of p53 can easily kill the cell line. Any suggestion will be...

09 October 2017 1,202 3 View

Good protocol for global DNA methylation measurement by LC-MS/MS method?

Hi All, I am starting to develop a LC-MS/MS based quantification method to determine the global DNA methylation level in cultured cells after compound treatment. I am following this paper right...

05 June 2017 6,027 3 View

Fluorescent polarization - strong fluorescent increase after adding protein?

Hi All, I am developing a high throughput fluorescent polarization assay against my target. It is a histone binding domain and the fluorescent probe is attached to a peptide. The protein domain is...

02 March 2017 8,446 26 View

Have anyone try growing protein crystals without gel filtration step?

Hi All, I was told that it is best practice to attempt growing protein crystals only after gel filtration column, but my protein is already quite pure after ion exchange column. I wonder have...

31 December 2016 9,469 6 View

How can I clean the waste generated from MTT assay?

Hi All, I have done tons of MTT assay lately for my project and have so many plates filled with the purple formazan solution (I used the SDS lysis method so no DMSO present). I just have no idea...

07 August 2016 5,805 3 View

Transfection reagent for PX459 (CRISPR-Cas9 gRNA vector)?

Hi All, We have problem getting good transfection of PX459 by Lipofectamine 3000 into A549 cells (I am not 100% sure, just that we don't get any editing with all 4 gRNA we designed based on...

07 August 2016 7,433 9 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View