Best growth medium for SK-N-SH?

11 November 2016 1 9K Report

I am currently trying to grow SK-N-SH cells and they are growing extremely slowly, much slower than any other cell line I have ever used.

I have been trying different formulations of media and am currently using DMEM with 10% FCS and pen/strep.

Does anybody use these cells and get good growth from them with maybe another formulation?

Zoe Allen

Do you have L-Glutamine or GlutaMax in the DMEM medium? Glutamine (GlutaMax is a more stable form of glutamine) supports the growth of cells that require a lot of energy and that divide rapidly. You can also try adding non-essential amino acids to the medium.

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