How to calculate a Master Standard Curve for qPCR?

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Cristian Ramos-Vera

https://www.bu.edu/iscf/files/2010/07/qPCR_Quant_Protocol_Guide_11322363_A.pdf

https://www.youtube.com/watch?v=pz54_p20lrI

Giorgio Medici

Thank you very much for your answer, but I'm trying to adopt a master calibration strategy where a single curve is derived from DNA standard measurements generated from multiple instrument runs. Thus, I would like to know the best method to get it, whether it is just making an average of intercepts and slopes from the different single curves or not.