Best coating quality, lowest operating cost & better reliability: MultiCoat or Praxair HVOF?

More Jonathan Durocher's questions See All

What are the factors that causes the sample to have higher efficacy at lower concentration?

We conducted an antibacterial study of a plant extract. Varying concentrations of crude extract were subjected to microbroth dilution assay. The result showed that only the lowest concentration of...

31 July 2024 8,666 3 View

Beta galactosidase assay in milk?

I am planning to measure the the activity of beta galactosidase enzyme using a standard kit to detect glucose production. The kit reagent is a mixture including horseradish peroxidase and...

12 June 2024 7,331 1 View

How to conduct/interpret power analysis for binary GLMM within-subjects with 2x3 independent factors?

Dear community, I can't seem to find any clear information on power analyses work so I'm throwing my question out onto the internet hoping for answers. :) Study Details I am doing a...

19 May 2024 9,214 4 View

Does TSO- salt in a 5% NaHCO3 solution turn the solution into a dark green color?

Here's the brief: 1. I synthesized alkyl gallates (ethyl gallate, butyl gallate, and amyl gallate) using Fischer esterification. 2. I used TsOH instead of concentrated H2SO4 as the catalyst. 3....

03 May 2024 6,737 5 View

Help on Modified Nanopore Sequencing to do Sanger-like sequencing for PCR amplicon from bisulfite converted DNA?

Hi, I'm planning to do sequencing using Nanopore. The main idea is to substitute Sanger Sequencing that usually used to sequence PCR amplicon from BCD (Bisulfite Converted DNA) using nanopore...

23 April 2024 6,637 0 View

Lyophilization of leaves, stem bark, fruits?

Is it recommended to freeze dry harvested leaves, stem bark, and fruits prior to trituration and extraction? I'll use the extracts for phytochemical analysis & in vitro bioassays. Thank you...

31 March 2024 5,920 4 View

Are nature-based solutions relevant to building a resilient society in the face of drinking water scarcity?

Although there have been a number of initiatives, the shortage of drinking water is still a problem for some communities. In this context, we can see that people's need for water is increasing as...

26 February 2024 5,285 3 View

How to remove DCU and its remaining traces from Steglich esterification?

Firstly, the compounds that I want to synthesize are alkyl gallates (ethyl gallate, butyl gallate, and hexyl gallate). The overall reaction: Gallic Acid + Alcohols + (THF, DCC, DMAP, 0 Celsius)...

25 February 2024 2,181 3 View

Has anyone worked with Science Paths - reportedly a book writing service?

Hello everyone, I receive many emails about different journals and publishers inviting manuscripts (some predatory, some not); however, received something new. Someone from Science Paths sent an...

12 December 2023 1,819 0 View

Achieving the SDGs by 2030: myth or reality?

According to a report by the Sustainable Development Solutions Network (SDSN) published in 2023, only 18% of SDG targets will be met by 2030. Should we expect a new global agenda?

26 November 2023 7,921 3 View

Adhesion strength of coating?

How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...

10 August 2024 942 3 View

How do I conformally do PR spin coating on trench structure?

I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...

08 August 2024 2,298 0 View

Why do i have a weak plasma and no coating during rf sputtering?

I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.

07 August 2024 4,526 4 View

Is there any machine to do real time pcr?

I want to know how do you make real time pcr solation ? is there any machine to make it? thanks for answering

05 August 2024 1,660 0 View

What is the reason I get 400nm nanoparticles using an extruder with 200 nm membrane for cell membrane coating?

I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And...

30 July 2024 3,294 3 View

Technology developments may it for whatever reasons developed eventually its benefits are multi-Disciplinatary?

Your comments

28 July 2024 2,822 2 View

Is it necessary to attach fluorophore at 3' end in MIC RT PCR machine for Dual hybridization Probe designed especially for genotyping assay?

I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...

28 July 2024 5,658 0 View

Can anyone help me with in providing the procedure for purification of GSTtagged protein by gst affinity chromatography without an automated machine?

Purification of gst tagged protein

25 July 2024 1,720 0 View

What is the main used curing agent for epoxy based coating?

I am trying to coating metal oxide into steel substrate, for better sticking planning to utilse epoxy based, Could you please suggest suitable curing agent for the coating purpose

25 July 2024 438 3 View

New and trending areas in the field of production technology using microorganisms?

Novel areas especially

24 July 2024 8,416 0 View

Vadim Verlotski

Woka Jet 410S from Sulzer Metco and JP5000 from Praxair are pretty much identical, and even compatible. Operating costs in both systems are due to powder costs and media costs (kerosene fuel, oxygen and hydrogen). Quality of the layers is hardly differ in both systems

Ali Feizabadi

Suggest the following paper:

https://www.sciencedirect.com/science/article/abs/pii/S0925838818307771