Hi everyone!
We typically record from rat acute hippocampual slices for LTP but I also need to expand my study into long term depression. After I ran a couple experiments, I completely forgot that you need to remove MgCl2 from your ACSF before attempting LTD. I see many different recipes out there for ACSF with LTD.
What has worked best for you?
Thanks in advance!
Dear Melinda,
LTD is very tricky to induce, especially with LFS. It works best in juvenile animals. DHPG is easier to induce.
It is not needed to remove Mg2+ from your ACSF.
see for example this paper https://www.nature.com/articles/s41598-017-01218-0#Sec12
I have tested many different protocols to induce LTD in slices from mature mice during my PhD. I had a look at my old unpublished data. The only protocol that worked was a 5 minute application of NMDA. See Article NMDA Induces Long-Term Synaptic Depression and Dephosphoryla...
DHPG, 1200, 900 2x 600 pulses at 1Hz all failed to induce LTD.
good luck
In our lab we can induce NMDAR-dependent electrical LTD even in old mice using this strong protocol: low frequency stimulation at 2Hz for 10min, repeated 3x with 10min interval (complete induction phase lasts 60min). This works with sharp electrodes as well as in a multi-electrode array (MEA) (both setups record from submerged slices). The ACSF is exactly the same as we use for LTP and contains MgCl2 (but we have also used MgSO4 instead of MgCl2). For more details, you can have a look at some of our papers:
https://www.ncbi.nlm.nih.gov/pubmed/21504782
https://www.ncbi.nlm.nih.gov/pubmed/21167950
Induction of mGluR-LTD with DHPG also worked in our hands:
https://www.nature.com/articles/nn.3342
https://www.cell.com/neuron/abstract/S0896-6273(15)00685-6
Best,
An
Actually, I've just got a nice LTD in mouss hippocampal slices with a 5 Hz LFS for 3 minutes.
Only problem is that the duration of the LTD is of about 6 minutes, while what I've found on literature is of 10 minutes-ish.
Has anyone tried this protocol and had a similira outcome? I'm also wondering what is the spike duration that you are using in your protocols?
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