Do you have a published paper which used the TPMT-ELISA method?

There are many companies which produce TPMT ELISA kit, but I need published paper which used the mentioned method for TPMT activity.

31 December 2014 824 7 View

Is there any published paper (clinical trial) which showed vitD effectiveness on cancer prevention?

There are a lot of published data that showed association between vitD deficiency and many disorders e.g. diabetes, metabolic syndrome, CVD, cancer,...

31 December 2014 3,254 8 View

BCQ22: Which is the main enzyme for activation of carcinogens? CytP450, Hydrolase, Acetylase, amin oxidase?

BCQs related to Cancer.

11 December 2014 3,205 0 View

BCQ19: What are criteria for lipid classification? Their chemical structure? Or physical properties?

BCQs about Lipids.

11 December 2014 5,997 0 View

BCQ4: Which type of fructose has higher glycolysis rate? oral ingestion or venous injection?

BCQs are Biochemical questions for education of the related students.

11 December 2014 2,790 0 View

BCQ11: Why is pH regulation important for human living?

BCQs about water.

11 December 2014 7,807 0 View

BCQ1: What is difference between prefixes Tri and tris and also di and bis in biochemical compounds nomenclature?

11 December 2014 2,480 0 View

BCQ3: Which monosaccharide glycolysis rate is higher?

BCQs are Biochemical questions for education of the related students.

11 December 2014 8,870 0 View

BCQ10: What are the main biological functions of water in human body?

BCQs about water.

11 December 2014 1,289 0 View

BCQ2: What is difference between a synthase and synthetase enzymes?

BCQs are Biochemical questions for education of the related students.

11 December 2014 8,374 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View

No mRNA increase in PCR after successful transfection in ARPE-19 cells ?

Hello, i am a bit curious about my results. I transfected the human cell Line ARPE-19 with a Plasmid containing a GFP tagged p53 (p53-GFP, Plasmid #11770). After 24h i checked under the...

18 February 2021 3,811 7 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View