In normal tubular tissue, renal tubular cells contain both functioning apical and basolateral membrane transporters. However, I am not sure in the cell culture model study that cells attach with the polyvinyl vehicle, their basolateral membrane transporters still works on exchange ions/organic substrates with the media or not. For example Na+ is rebsorbed into cells and transports back into bloodstream. Though cell culture contains not interstitial tissue or blood vessels, reabsorbed Na+ will be stagnant in the cell or secretes back to environment using basolateral Na transporters? I appreciate for every comments
Hi Thasinas, have you tried coating Transwells with tubular cells on top?
Then you can monitor whatever the tubular cells transport by assaying the lower chamber.
Hi, Thasinas!
Well, if you grow cells on glass / plastic, the basolateral membrane has difficult time. For example, monolayers of M1 cells from mouse collecting ducts form so-called "domes", which are essentially sites where they detach from substrate because of the increasing volume of absorbed solution. However, if the same cells are grown on permeable filters, the whole system works fine.
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