Hello Di,

The cell line looks good. But the think is a variety of DMEM is commercially available. So you have to be sure about the exact combination. I used DMEM that contains 4.5 gm D +glucose and without sodium pyruvate. apart from that you have to use glutamax additionally, as glutamine get degraded as time passes. If you can say the catalogue number of your DMEM it will be good. We generally used DMEM High Glucose(Thermo Fisher Sc. Catalogue# 11965-092). Good Luck

Thanks,

Prakash

The morphology is correct. Please be sure that you do not allow the cells to get to confluent . This could make them  change morphology and sometimes phenotype.

Thanks

Not my area but corneal endothelial cells grow large apparently to fill a void, due to apoptosis of neighboring cells.

They look pretty similar to these HEK293 at Elabscience, just more dense: https://www.elabscience.com/p-293_[hek_293]_cell_line-58964.html

Also pretty similar to this lot of HEK293T at ATCC: https://www.atcc.org/products/all/CRL-3216.aspx#characteristics

Or in this publication: Article Hsp70 Interacts with the Retroviral Restriction Factor TRIM5...

It would be interesting to see how they look at different densities during growth.

Different batches of HEK293/HEK293T cells can have slightly different morphology and tendency to grow as islands. According to the literature, the morphology and the strength of attachment can vary with different lots of FBS. The density after dilution and detachment/attachment propensity will also affect the final patterns at high density.

The batch of HEK293T (tsA201) I am using is less clumped together at lower density but eventually they all can form a dense layer.