I am working on Bacteriophages where i do the plaque assay to estimate the phages titer or viability of phage.
Background: i applied the phage to plant and then do the plaque assay after different time point such as 1,4,7 and 21 days to know about the phage retention in plant.
My question is
1) There any other method to know about the phage viability other than Plaque assay?
2) Research also recommend the qPCR for phage quantification. But the phage DNA may present in plants even if it is not viable. so, what is the other method for this.
First way - plaque assay. If viability is a ability of phage for productive infection, so its classical way. Second way - selective potentiometry. It s based on fact that when phage infects bacterial cell and inject his nucleic acid into the cell some ions effluxes fromyou can meature it concentration and judge about productive phage infection cell. That is to ways that I can recall
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