I have 2 question regarding the topic.
1. What is the method for bacterial DNA extraction. The complete methods with buffer and other solutions.
2. How to perform bacteria identification using 16s rRNA sequencing. What are the complete protocol for this?
I have gathered some information on these but I want know more in case there are some part are missing.
Thanks in advance.
Hi Sharifah
For the bacterial DNA extraction, there are so many methods already available from the conventional to using kit.
Here attached one of the protocol for bacterial DNA extraction.
Beside this, you can also use kit, as example:
1. https://www.thermofisher.com/nl/en/home/life-science/dna-rna-purification-analysis/dna-extraction/genomic-dna-extraction/bacteria-dna-extraction.html
2. http://www.sigmaaldrich.com/life-science/molecular-biology/dna-and-rna-purification/bacterial-genomic-miniprep-kit.html
3. https://nld.promega.com/resources/protocols/technical-manuals/0/wizard-genomic-dna-purification-kit-protocol/
For 16s rDNA, you can find in this paper: http://link.springer.com/article/10.1007%2Fs10529-005-4688-z
Good luck and success
Best,
-Fean-
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