Hello,
Hope everyone is doing great. I have synthesized an antimicrobial peptide gels. Now I need to perform antibiofilm assay to check its biofilm inhibition potential.
There is this paper I am following. They grew biofilms on silicon wafers modified with gel and then incubation. I don't have silicon wafers.
Could someone please tell me that is there any alternative? Can I grow biofilms in 12-well plate already modified with gels?
Secondly, why they grew biofilms on silicon wafer? Is there any technical aspect related to SEM analysis afterward?
Regards,
Zeeshan
Phil Geis
Thanks for your response.
Biofilm formation protocol would be the same as any other. Because bacteria need solid surface to grow which silicon wafer provide.
In my opinion, I can modify the well plate with gel and then establish the biofilm on it.
I want to ask, is there any technical aspect of establishing biofilms on silicon wafer regarding the SEM analysis? I mean would it be easy to do SEM analysis when biofilm grows on silicon wafer?
Thanks Phil Geis for you response,
I have never thought about this aspect. Well, It really seems natural as it mimics the real life problem in case of biofilm formation on medical equipment and devices.
But when it comes to SEM analysis how are you gonna prepare sample? And incase of silicon wafer, you just cut a small piece and its ready to be analysed along with its surface.
Maybe that is the only reason of establishing biofilms on it.
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