May I know is there anybody dealing with DNA/RNA extraction from baby stool by using kits? Any advice in between QIAGEN vs Zymo? Or other non-kits related methods which you feel is workable and effective for downstream?
As I noticed, there is differences between extraction on adult and on baby's stool due to the composition. Any advice is welcome. Thanks.
Hi Clara.
In our group we extract baby stool sample with the same protocol that adult stool samples. We use the SOP 07 that recommend the International Human Microbiome Standards. The SOP 06 it's a QIAGEN modification that works similar than the SOP 06.
I am attaching you both protocols if you want to check them.
Best
Hi David, thanks for your reply and the protocols, is the quality and quantity of the DNA/RNA extracted good? For me, I am using the protocol for adult stool samples, but the concentration are really low
The quantity is really good. For adult samples we have 1000 ng/ul of DNA and for baby samples we obtain 500 ng/ul, for us this amount is enough. The quality is similar for both between 1.6 and 1.8
Oh wow, although I just started, I hardly get above 100 ng/uL for DNA and RNA concentration.. Any important step to look out for during the extraction? May I know what downstream are your group looking at?
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