Azadirictin molecules from neem seeds not separated completely, other components produces need separate, plz suggests better options
Dear Vipul ,
I suggest using TLC technique to determine what type of solvent you should be using in extraction . After that you can using silca gel to get fully separte.
Goodluck
Please read these articles. Thank you
Agree with Ghassan Al. Samarrai.
Thank you Sir
I have used Schrödinger (Glide) for docking
13 July 2024 3,003 1 View
Hi, I have isolated exosomes from mouse epididymis using SEC in 300ul eluate (PBS) volume. I intend to concentrate the sample in order to increase the protein concentration (for LC-MS/MS) but I am...
29 August 2023 9,013 0 View
Hello I have a query regarding calculation of Projected DOS, using Quantum espresso (QE). We are studying about a oxide structure, and as it is spin polarized calculation we are using fully...
05 March 2023 8,180 0 View
Hello!!! I am a novice in quantum espresso, so I need your help in this regard. We are currently working on the 1x1x1 Ni3Mo (001) surface, to find out the change in the Gibb's free energy when...
25 January 2023 7,794 2 View
Hello !! I am a novice in Quantum Espresso. Please help me out in the following problem: We are trying to optimise Pt(111) surface , it is important to note that even though the attached files...
15 December 2022 6,786 5 View
Somebody kindly send template of World Journal of Engineering.
13 December 2022 4,752 4 View
I want to convert scale in a microscopy image 200x to micrometer scale
21 July 2022 9,730 1 View
We want to quantify an IHC image for specific expression of proinflammatory cytokines using image J or any other academic software
25 May 2022 7,265 1 View
Dear researchers, I wish to understand the role of grain growth inhibitor in controlling the microstructure of the ceramic pellet. How it controls the growth? Also, I wish to know the grain...
15 January 2021 4,196 3 View
what are the invivo screening methods for anti TB drug?
25 December 2020 901 1 View
I'm working on camelina seed sterilization and have some doubts about the process. I followed a protocol used by my lab mate, but I'm unsure about a couple of things: Is it safe to use 10% bleach...
02 August 2024 1,178 3 View
I have been following Rivero et al 2014 protocols, but I am not sure when I should stop watering because my plants are completely yellow but the siliques are not 80% brown. Any recommendations?...
30 July 2024 2,301 7 View
Hello all, Recently as part of a research project at university, we have been studying the effects of light and temperature on eucalyptus seed germination. In our initial run of trials we...
24 July 2024 8,850 4 View
Hi, Kindly guide me that how many cells of HEC1A & HEC1B Cell lines should I seed for Wound healing assay and which plate type is recommended 6, 12 & 24?. Articles suggested mainly 24...
20 July 2024 4,143 2 View
I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...
11 July 2024 5,669 5 View
I have protein percentage and Seed yield per hectare data, how to calculate protein content kg per hectare.
11 July 2024 9,045 4 View
I am currently testing the effect of bacterial filtrates on cancer cells , after seeding the cells I tested the bacterial filtrates against them , and got images of all 96 wells using inverted...
10 July 2024 7,145 2 View
I seed my cell (KG1a )in opti-mem media for transfection (without lipofectamine as control ) and after 2 hours I add FBS 10 ul in each wells all my cells after 24h in control wells or with...
07 July 2024 407 0 View
I have attached pics of the SH-SY5Y cells within a seeded plate after reaching >80-90% confluence. Are these dark clumps of multiple growing cell layers? Or dead cells? Any clue? many thanks.
01 July 2024 8,361 3 View
Hi everybody, i need to pick your brains: i work with an adhesive fish cell line which forms a konfluent monolayer. I want to test an adsorbant substance, therefore i want to saturate the cell...
30 June 2024 9,300 0 View