Autoflourescent vs Compensation problems?

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Why the magnitude of trend using Sen's slope and linear regression after data smoothing using exponential smoothing has statistical differences?

Dear Scholars, I'm working on a trend analysis of hydroclimate time series data. I attempted to compare the magnitude of the trend using Sen's slope and linear regression after smoothing the data...

19 October 2023 6,715 3 View

How to calculate missing daily precipitation data by Visual Basic Codes in excel?

I am preparing precipitation data for HEC_HMS hydrological model . There are some missing daily precipitation (rainfall) value in the dataset. Can anyone suggest me how can i fill using a method...

28 November 2022 6,751 3 View

Can satellite images estimate channel morphology change accurately?

Are there appropriate satellite imagery What are tools for channel erosion and deposition assessment

28 March 2022 4,113 8 View

Which material model is best in simulating an embankment dam that is supported by a stone column using PLAXIS 3D?

In simulating an embankment dam that is supported by a stone column using PLAXIS 3D, the behavior of soil and stone column materials may be approximated to varying degrees of accuracy by the...

20 January 2021 2,846 4 View

How can i make my gating strategy consistent enough during Flowjo analysis? Can anyone help me?

I am analyzing my cells of interest, such as B cells, when i apply gates from one sample to another, am facing the applied gate in the second sample seemed that it is missing some populations....

28 November 2020 2,669 2 View

How to deal with autoflourescence when you have a panel with full antibodies?

We are analyzing T cells in response to X antigen, and it seems there is an authoflourescent after compensation has been done. I was told that in order to elilminate autofluorescence i have to...

09 September 2020 5,326 4 View

Why is that a positively expected variable happen to show a negative coefficient in panel data?

For example GDP per capita is expected to affect positively export diversification. But I keep obtaining negative and significant coefficients in fixed and random effect models.

23 August 2020 9,079 14 View

In the under resourced countries, what do you think are the top five health issues that Artificial Intelligence (AI) can help to address?

In a world where connectivity is destiny, Artificial Intelligence is our torch moving forward. As in many other sectors, AI is playing a huge role in reshaping the healthcare system. I would like...

24 May 2020 295 0 View

Are non classical monocytes are negative for CD14?

Hi guys, Am defining monocyte subsets. And classifying then to classical, intermediate and non classical. But when i am gatting non classical the CD14 expression seems smear up negative. What i...

15 April 2020 3,904 0 View

Why NH4CL not completely lyse Rbcs?

Am optimising my protocol using NH4CL based lysis. Which I prepared as follows:- 1. NH4Cl = 1.66g 2. KHCO3= 0 167g 3. Na2EDTA= 0.006 in 200 ml of distilled water and I freeze and use. But when I...

06 January 2020 8,565 0 View

Why my unstained, isotype (IgG) and positive samples (U87 cells) have same histogram results?

Hi, I just want to ask regarding flow cytometry method for indirect staining of U87 cells (Glioblastoma) as I'm still quite new in using this method. My objective is to observe the expression of...

23 March 2024 8,883 1 View

How do you deal with the right shift of histogram in imaging?

Good Day I am currently reviewing concepts, and practicing using a epifluorescent microscopy. I often notice that there is a right shift in the histogram of my images. I would like to ask/seek...

18 August 2023 3,438 0 View

Interpreting the results of a GAMM model and plotting the estimated effect?

I am trying to model the visitation frequency of bird to a plant species along an elevational gradient and two season. Moreover I have the number of flowers each observed plant had. My response...

02 August 2023 8,342 2 View

What is the histogram for the normal distribution of systolic blood pressure in the US?

I cannot easily find the data about blood pressure expressed in the normal distribution histograms I am best aquainted with. Where can I find them?

22 July 2023 1,322 1 View

If I only get a fastaq file from my whole plasmid sequencing order does it mean that it failed?

Hello, I submitted two samples for whole plasmid sequencing. I usually get a fasta. fastaq, the histogram and the consensus file. This time they only attached the fastaq and the histogram file....

23 May 2023 8,375 3 View

How to plot a histogram with MD simulation output files?

Please I need help, i completed a MD simulation and i want to plot this kind of histogram (the one I have attached). Which software can I use?? Also, what tools can I use apart from VMD to analyze...

21 March 2023 4,747 3 View

Can I use Chi-square test for normally distributed data?

Dear Experts, Can we use the chi-square test (which is non-parametric) on normally distributed data? A follow-up to this is.. can both parametric and non-parametric tests be performed on the same...

19 March 2023 9,933 8 View

Which tool use to plot horizontal histograms as shown in the attached figure?

These types of figures are required in reaction kinetics modeling work. #figure #plot #Histogram #reaction_kinetics #chemical_kinetics #combustion #combustion_kinetics

29 January 2023 481 7 View

Hello, can anyone guide me on how to plot a dose volume histogram (DVH) with Excel values (not DICOM) using MATLAB or Python?

If you know a software for drawing dose volume histogram (DVH) using numbers in any format other than DICOM format, it can be helpful. thank you.

21 December 2022 3,660 13 View

How to calculate the total cellular fluorescence intensity using CytoFLEX Flow Cytometer?

I have the dot plot and the histograms of the samples after the treatment with the fluorescence-labeled nanoparticles.

20 November 2022 1,256 0 View

Muhammad Ali

I suggest you follow similar query asked on RG here : https://www.researchgate.net/post/Any_advice_on_Auto_fluorescence-Flow_cytometry-Free_Channel_compensation