Henok Andualem

6 Questions 5 Answers 0 Followers

Questions related from Henok Andualem

How can i make my gating strategy consistent enough during Flowjo analysis? Can anyone help me?

I am analyzing my cells of interest, such as B cells, when i apply gates from one sample to another, am facing the applied gate in the second sample seemed that it is missing some populations....

29 November 2020 2,717 2 View

Autoflourescent vs Compensation problems?

Can anyone please see the below histogram? I had difficulty with the double-positive population.

03 October 2020 790 2 View

How to deal with autoflourescence when you have a panel with full antibodies?

We are analyzing T cells in response to X antigen, and it seems there is an authoflourescent after compensation has been done. I was told that in order to elilminate autofluorescence i have to...

10 September 2020 5,362 4 View

Are non classical monocytes are negative for CD14?

Hi guys, Am defining monocyte subsets. And classifying then to classical, intermediate and non classical. But when i am gatting non classical the CD14 expression seems smear up negative. What i...

16 April 2020 3,939 0 View

Why NH4CL not completely lyse Rbcs?

Am optimising my protocol using NH4CL based lysis. Which I prepared as follows:- 1. NH4Cl = 1.66g 2. KHCO3= 0 167g 3. Na2EDTA= 0.006 in 200 ml of distilled water and I freeze and use. But when I...

07 January 2020 8,608 0 View

How to incerease the number of events during whole blood assay?

Hi guys, am working on by flow cytometry analysis of DC and B cells using whole blood assay. Which means i stain , then lyse the RBCs and acquire. However, i couldnt find >400,000 events. I was...

02 January 2020 4,068 4 View