How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

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03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Please I want to know the method for measuring bile salts in bird how I do this in lab please help me to know the method?

I need to the required sample ,reagents and steps

10 December 2020 7,087 1 View

ERP studies: What criteria do you use to decide about inclusion/exclusion of data sets?

Do you know papers that discuss this subject? We are looking for objective methods to decide about inclusion/exclusion of data sets. For example: Bootstrap Signal-to-Noise Confidence Intervals: An...

03 September 2020 8,238 3 View

Do you think peer assessment is valuable to the assessment process, or learner progress?

Peer assessment helps students to be more objective about their own learning and it can assist learning, it is argued. Its worth to student evaluation would perhaps be questionable too.

23 April 2020 3,745 3 View

Is it better to use base e (LN) or base 10 (LOG10) when making estimates of body mass from linear measurements?

For linear regressions between linear measurements and body mass, which transformation is better to use? base e logarithm (LN)? or base 10 logaritm (LOG10). Apparently they do not make such a for...

08 April 2020 5,201 12 View

What is this structure in the laparoscopic image?

This is an operative image during laparoscopic surgery for hiatal hernia. What is the structure in this image referred to it by an arrow? Is it thoracic duct or another structure?

15 February 2020 7,770 14 View

What is this structure in the laparoscopic image?

This is an operative image during laparoscopic surgery for hiatal hernia. What is the structure in this image referred to it by an arrow? Is it thoracic duct or another structure?

01 February 2020 4,611 11 View

How to differentiate Peribiliary gland cells from Bile duct cells?

Hi, Can someone suggest a good marker to segregate Peri-biliary gland cells from cholangiocytes lining cells? Also, a good marker which is expressed in activated PBG cells during bile duct injury

11 December 2019 6,101 2 View

Is it possible to do Chi-square test for replicates?

In the attached file. I have something like a contingency table. But in every group, there are two biological replicates instead of one. How can a chi-square test be used in this situation and if...

10 November 2019 6,672 11 View

Mouse islet isolation without pancreas perfusion?

Is there a current/reliable way to isolate mouse islets (keeping their morphology and viability for culturing in vitro) without perfusing the pancreas through the bile duct?

14 September 2019 3,577 1 View

Mouse islet isolation without pancreas perfusion?

Is there a current/reliable way to isolate mouse islets (keeping their morphology and viability for culturing in vitro) without perfusing the pancreas through the bile duct?

08 September 2019 6,824 1 View