Spectrometric method

Sir can you elobarate your answer why spectrometric method the substrates and chemicals used in titrimetric method are very economical when compared to spectrometric method

Hi first i couldn't catch your question it's not clear? some how i got your doubt, in titrimetric method you may use sample conc in mg or gm, but in spec you can analyse sample even in micromole or nmole also volume required for analysis also very low.

there is a limitation in using the spectrophotometer in lab and the other thing is substrate used for titrimetric assy is olive oil but in case of spectrophotometer we need to use para nitro acyl esters which are very costly what about the reliablity of results in both method ?

My second doubt is among the para nitro phenyl esters used for assy which is the best substrate ?

Thanks for the response sir

Titrimetric method is officially referred in pharmacopoeias and it is a basis for definition of international, commonly used FIP unit. I agree, the method is tricky and requires some experience. I've also heard that the spectrophotometric method can give unexpectable results because the substrate is very unstable.

Thanks for the reply madam madam the titrimetric method of estimatation of lipase requires large amount of reagents and i agree it requires experience.I have another doubt madam if we are using emulsified substrate in the method it increase the stability and hence can we get correct results ?

IMO emulsified substrate mimics natural process of lipolysis in duodenum and small intestine, where lipids form emulsion due to cholic acids presence. For that reason the emulsion droplet size is crucial for good recovery in titrimetric method. It is also a good practice to use lipase reference std to control the recovery of your assay.

You can use manual titration with aid of pH-meter or automatic titration with titroprocessor applying pH-stat titration mode.

Thank you very much for your valuable suggestion madam it will definatly help me in my research madam.