I have tested radiosensitivity of drug-treated cells and radioenhancing effect of heavy-metallic nanoparticles for a while. It seems that clonogenic assay, gamma-H2AX assay, comet assay (or DNA ladder) and micronuclei formation assay can be used for the evaluation. Does anyone know which one is more validate or any notes of each assay for different applications? Thanks.
If you want to detect SSB and DSB, you must use comet assay immediately after the treatment (such as radiation) and for assessment of DSB gamma H2AX assay is the best method.
Time of the examination after a treatment is very critical.
Clonogenic assay is the gold standard method for evaluating the radiobiological cell death (cell stops deviding).
H2AX is good for tracking the radiation field and demostrates the DNA damages, but doesn’t have any value more than that. Even very low radiation does can make it positive but cells are able to repair most of these damages.
So it depends on your purpose.
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