Does anyone have experience or insight on how to proceed with this method? Specifically, I’m trying to understand how MIC, MIC₅₀, and MIC₉₀ values were determined using absorbance data, as described in the following statement:
"MIC values were determined based on the concentration of extract at which the absorbance was less than or equal to the negative control. The MIC value results (concentration that caused 50% and 90% inhibition of bacterial growth) were determined."
Any guidance or explanation would be greatly appreciated.
Harshani Dilrukshi Alahakoon MIC is basically the lowest concentration of an extract at which the bacterial growth is completely inhibited and with absorbance it is indicated by an absorbance value (typically at 600nm) equal to or lower than the negative control (media only). Whereas the MIC₅₀ and MIC₉₀ represent the concentrations that inhibit 50% and 90% of bacterial growth, respectively, and are calculated by first determining the percent inhibition at each concentration using the formula: % Inhibition = 1 − (Abs_sample − Abs_blank) / (Abs_control − Abs_blank) × 100. These values are then obtained by plotting inhibition vs. concentration and identifying the points where 50% and 90% inhibition occur, either directly or through curve fitting plot on origin or graphpad prism.
Shashank Kashyap,
Thank you so much for your answer, and it would be helpful for my analysis.
Harshani
- Badges
- Science method
- Similar topics
- Biological Science
- Ecology