I plan to perform the Fluorescence polarization experiment. I have some FITC peptides ordered from the company with purity > 95%.
My peptide is 15-20 a.a long and very basic with pI ~12.
I read that most people dissolve it in DMSO.
Can I dissolve my FITC peptides in water?
Thank you!
You can use a peptide solubility calculator to predict its solubility:
https://pepcalc.com/peptide-solubility-calculator.php
Since it is composed of several charged residues with a pI ~ 12. It should be soluble about 3 pH units from its pI since it is uncharged when pH = pI.
Should be soluble in water or Tris pH ~ 8. Use autoclaved sterile water and filter the peptide.
If the peptide was composed of a lot of nonpolar amino acids then it wouldn't be soluble in water.
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