Aside from pIC333 and pAW016 are there any transposon vectors that make mutant libraries in gram and bacteria?

13 February 2014 1 221 Report

I am looking for a vector system that I can use to make mutant libraries of Bacillus cereus. I have tried pIC333 and pAW016 but they did not provide the randomness I expected. Is there any other vector I can try?

Gumpanat Mahipant

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1352254/

Try this one. There is related vector that workable in cereus as well as this paper below.

https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-016-0492-9

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