I am working on fungi, old specimens from herbarium, as the specimens are really old some times it is not easy to get the extract. I need some advice on adjustment the method; for example leaving the lysed tissue over night in room temperature or even water bath.
It is good to know if some one has got similar experience.
Let the lysed material overnigth around 40-45 C. But anyway, several times the results are incomplete sequences with several gaps or incongruences, which are hard to align.