When performing imaging experiments with onion protoplasts, I noticed what seems to be some kind of cellular body that exhibited fluorescent signal across multiple channels, even in my samples that should not exhibit fluorescence. Is this a known issue with imaging onion protoplasts, or potential something wrong with my imaging settings?
Same green spot - are you using the same kind of instrument?
https://www.researchgate.net/post/Whats_the_best_way_to_measure_immunofluorescence_in_a_large_image
Thank you for sharing your interesting picture. I have never seen this and really have no idea. Except, is it a proplastid (or developing chloroplast) that might contain an auto-fluorescing pigment like chlorophyll? You would probably need TEM to see if it has recognizable internal structure. Good luck with your project and let us know if you figure it out. Peter
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