Why is WST-1 assay giving inconsistent result?

i want to determine the cytotoxicity activities of some drugs with WST-1 assay using 293T cell lines in 96 well plate. I usually seed 10,000 cell per well and incubate for ~48 hours. I expected...

01 February 2021 8,554 3 View

Can you do a research collaboration with me on building feeling into telepresence devices?

We need to move from virtual reality to real world again. The research aim at making individual working on a robot at a distance to feel what the robot feel order than by vision and tactile...

08 June 2020 2,575 6 View

Nanoparticles in lubricants ?

How is it that we propose nano particles in lubricants, for instance, engine oil. To what extent does the particle size in relation to viscosity affect the metal surfaces. Is it a trade off due...

03 February 2020 7,385 4 View

Please can anyone assist with MATLAB codes for sensitivity based finite element updating?

The updating is mainly to reduce the descripancy between experimental and numerical modal frequency

10 August 2018 2,262 3 View

What is the limit of cyanide concentration in the soil that is not a threat to the environment?

25 August 2016 8,852 3 View

Validation of mathematical/analytical Models

The acceptable practice is to validate a model used under any study. What validation methods are available and would we confirm one superior over others?

01 January 1970 5,708 19 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Is anyone else having a similar situation?

I'm looking for the genetic sequence for the TAR region of HIV. If I put Transactivator Response Region, and HIV, and "genetic sequence: into a browser...is it not unreasonable to expect to be...

10 February 2021 658 5 View

Can HIV drugs will be a provisional or recurring option against COVID-19?

Can HIV drugs will be a provisional or recurring option against COVID-19?

22 December 2020 7,872 2 View

Can the formation of drugs against coronavirus also be useful against the Influenzas virus or HIV?

Can the formation of drugs against coronavirus also be useful against the Influenzas virus or HIV? This is because these viruses shared many common proteases for viral replication.

22 December 2020 6,508 6 View

Why accuracy over each k-fold corssValidation differ alot?

I have a data set with 91 Obervsation with 700 features. I have reduced the dimension of data using PCA? then I split the data set into 70/30. After that applied kfold =10 cross-validations on...

04 December 2020 1,259 4 View

What is expected from a PhD ?

PhD is considered as the highest degree one can get from the university and also is recognized worldwide. What is expected out of a PhD degree, the holder and the providing institutes ? What are...

11 October 2020 9,412 13 View

Please some paper about Adaptive immunity against viral infection???

26 April 2020 1,999 10 View

Can someone send me the list of RNA dependent RNA polymerase inhibitors?

It would be very helpful if someone can send me a link or text containing the list RNA dependent RNA POLYMERASE (RdRp) inhibitors.

20 March 2020 5,707 4 View

What is the appropriate statistical treatment to be used if our study aims to identify the level of knowledge of college students with regards to HIV?

We will use a survey which contains close-ended questions.

14 March 2020 9,676 3 View

How would I determine if a protein (PD-1) is necessary for establishment of latent infection by a virus in a CD4 memory cell line?

If I were to plan an experiment to deduce whether or not PD-1 is absolutely necessary for HIV to establish latent infection in CD4 memory cells, how would I go about doing this? More...

09 March 2020 7,774 2 View