We're conducting an experiment to develop an assay to measure phosphate. At this stage we need a simple experiment that we can do to show an increase in phosphate. Do you have any suggestions?
First thing that comes to mind would be bone meal fertilizer. This is a slow release fertilizer made of animal bones. Phosphate concentrations would surely increase over time if this was added to water.
I do not understand, if you need to measure the phosphate or design an experiment in which the phosphate concentration increases with time. to measure phosphate there is a colorimetric reaction with molybdate and reducing agent very simple, I do not know if that helps.
The same situation to Lisandra. It depend the goal and I agree with to measure phosphate there is a colorimetric reaction with molybdate
Determination of the percentage of phosphorous (as P2O5) in fertilizer by using gravimetric analysis. The gravimetric analysis is based on the precipitation of phosphorous as MgNH4PO4.6H2O from a solution containing monohydrogen phosphate ion, ammonium ions and magnesium ions.
If you are measuring phosphate in solution perhaps try: malachite green (MLG) assay of phosphate or Bromocresol purple or ascorbic acid-molybdate. However they all have different dynamic rangers, so choose the method (if workable) that is most appropriate for you range.
All the best
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