What is the mol. weight of GFP-11 tag?

Hi, I am trying to find out the mol. weight of GFP 11 because it is tagged to my protein of interest which is 17kDa. GFP 11 has been tagged with my protein of interest while GFP 1-10 is tagged...

09 December 2020 1,863 1 View

How should I store the milk sample in RNAlater?

I want to extract RNA from human breast milk. How should I store the milk sample in RNAlater? And after RNA extraction how can I store the RNA?

01 December 2020 7,183 4 View

Which is the best stripping method for proteins having molecular weight 90kDa and above?

Hi, I would like to know which is the best stripping method for proteins having mol. wt. around 90 kDa or more. I am using human samples. I used 0.5M EDTA stripping buffer by boiling PVDF...

08 September 2020 5,815 2 View

What is the best solvent for dexamethasone so that it does not get toxic for the cells?

Hi, I am using C2C12 cells. I need to treat them with dexamethasone. I would like to know which is the best solvent to dissolve dexamethasone so that it is not toxic to the cells? I am using dex...

22 June 2020 4,870 6 View

How to build alignment files for homomeric and heteromeric proteins using Modeller?

I am building protein structures for a list of query proteins via homology modelling using Modeller. In some cases the template is a homomeric protein and in others its a heteromeric protein....

01 April 2020 6,576 3 View

How to correctly standardise migration assay using HEK 293T cells, without the use of matrigel in transwell?

So, I have been trying to quantify the migrated cells through transwells without using Matrigel (because I just want to quantify the natural migration rate of HEK 293T). 1. I plate them on the...

19 February 2020 7,123 1 View

How to measure vagal tone index?

07 October 2019 9,748 3 View

What could be the most reliable or accurate method to quantify the amount of E-waste generated in a developing country?

The methods of estimation of E-waste are both complex and diverse. Different models available in the literature show different results. The availability of information on E-waste quantity...

03 April 2017 5,499 1 View

How to simulate water Platinum interaction in LAMMPS?

I am working on water drop simulation on Platinum surface using LAMMPS. EAM is used for Pt and TIP4P for water, but how to model the interaction between water and Platinum using LAMMPS?

06 June 2016 6,340 2 View

ER stress induction in Hap1 cells?

26 April 2016 7,145 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Is stone a sustainable material? Is it a sustainable approach to use natural stone waste in architecture?

I'm designing a zero-waste restaurant and considering using marble waste to make some tables and a bar countertop. My clients think it's not "sustainable enough" and I would like to have more...

01 March 2021 4,326 7 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

What role should journals play in leading academic fields?

A recent paper by Yeung and Thomas (2021) on journals in event management opens up an interesting debate on the role of journals as academic fora. They demonstrate that event management publishing...

28 February 2021 3,494 6 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View