Hi all,
I am looking for yeast selections that result in adaptive mutations appearing only in a single gene. That is, if I pick single colonies that survived this specific selection, all or nearly all of them will have the adaptive mutation/s in the same gene. 5-FOA selection, for example, results in adaptive mutations mostly in the URA3 gene. The same for canavanine and CAN1. I am looking for more such examples. I am not looking for solutions that involve genetic engineering (i.e., no selection for revertants of introduced conditional deleterious mutations etc.).
Thank you
Here is another example. TRP-1 marker you can use 5-fluoroanthranilic.
Great, thank you Hanna. Still, looking for more such examples.
Daniel
There is another example that I know of, however, I strongly advise against using it since it uses toxic mercurial compounds. met15/met2 mutants are resistant to methylmercury. 50% out of cells resistant to methylmercury require methionine and 84% of those are mutated in met 15 while 16% are mutated in met2. Find the ref below:
Article Characteristics and Relationships of Mercury-Resistant Mutan...
Thank you Hanna,
This is a great example. I wasn't aware of it.
Daniel
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