In my recent project, I needed to separate nanoparticles from the flowing samples. But I found it so difficult to do that. Who has experience on this topic? Any suggestions are welcome! Thanks so much~
I use flowcam for analysing microparticle mixtures, but nanoparticles would be a challenge.
what would be the size range of your particles? will ultracentrifuge help to separate bigger particles ?
(Capillary) electrophoresis; ultracentrifugation; size exclusion chromatography.
If your nanoparticles is a magnetic material, you may use any strong (better asymmetric) magnetic field.
Thanks for all of you! The operated particles size from sveral nanometers to two handred nanometers, and have no magneitc features. This is the challenge.
At that size range you might want to give field flow fractionation FFF methods a go. Any gradient can be applied to enhance separation, but thermal FFF is the most common.
Thanks for remindering me of the FFF methods, which have triggered many fresh applications and new micro/nanofluidic strategies for nanoparticle sorting.
you may calculate by Stokes law how long to centrifuge to separate larger particles down to a specified size. You may use reversible depletion flocculation to separate the larger particles.
Hi, Titus, thansk you very much! It's my first time to hear about this method. Could you provide me some references about the Stocks law based centrifugal calculation? thanks again!
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