I want to design primers to NGS in the illumina. Are there restrictions on the size of the overlap section or at sea level or sequence primers previously paved section?
Would appreciate help and tips!
maybe some of these links will be helpful for you
http://nextgen.mgh.harvard.edu/CustomPrimer.html
http://tucf-genomics.tufts.edu/documents/protocols/TUCF_Understanding_Illumina_TruSeq_Adapters.pdf
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