Are there Hif1a antibodies that work reliably for immunofluorescence on frozen sections?

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How does public perception of vote buying affect voter behavior and participation in Ghana?

This question explores the impact of public perception of vote buying on voter behavior and electoral participation in Ghana. It seeks to understand how voters' views on the practice of exchanging...

19 July 2024 8,004 0 View

How to change reference list (muilitple pages) from APA to Harvard?

Hello, I am looking for a tool that will do a bulk conversion of references from APA to Harvard. There are over 20 pages of references, so I would like to avoid doing them one at a time. Thank...

07 July 2024 2,003 2 View

Could anyone recommend antibodies to TET2, TET3 and DNMT3B for bovine?

Can anyone recommend polyclonal or monoclonal antibodies for Tet methylcytosine dioxygenase 2 (TET2) Tet methylcytosine dioxygenase 3 (TET3) DNA methyltransferase 3 beta (DNMT3B) Ideally, they...

27 June 2024 3,557 1 View

What is the materials composition of automotibles?

What share is made of steel? Plastics and glass? Aluminum? Iron?

15 June 2024 7,246 2 View

How do I change my email address?

Today is my last day working at Missouri Department of Conservation. I have a new email address, and I don't know how to change it in my profile, so that I will continue to receive ResearchGate...

13 June 2024 8,970 2 View

Does anyone know where one can buy 8161 glass capillaries for making patch pipettes?

Warner used to sell 8161 glass, but Harvard Biosciences bought Warner and doesn’t appear to stock it anymore

04 June 2024 5,688 2 View

What are the most appropriate variables for measuring profitability of Robusta Coffee production by smallholder farmers using panel data?

Dear researchers and academicians. I am kindly requesting for your contributions to the above question on proxies for profitability of smallholder coffee production. Thanks so much and kind regards.

04 June 2024 722 0 View

How can I get a list of my publications in Research Gate and the number of Reads and other stats?

I would like to easily track which of my articles are getting reads and which are not without clicking on articles one at a time.

22 May 2024 9,047 3 View

T test; paired, student or independent?

Hello all: Any help appreciated. I am running an intervention with students in a esl environment. I want to compare knowledge and awareness of the topics pre and post intervention. The issue is...

14 April 2024 2,237 2 View

Preventing loss of fixed cells after washes with PBS?

I'm unable to currently continue the work on my thesis due to losing a huge number of cells in wash steps (up to 75% after 2 washes). I'm using 2 cell lines Kasumi-1 and Molm-14. I'm working in...

09 April 2024 3,990 5 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

What are those black/hollow structures?

Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...

21 July 2024 1,044 1 View

How to perform immunofluorescence on suspension cells?

Some time ago, I wanted to perform immunofluorescence on the S protein of mouse CD4 T cells using a Leica super-resolution microscope. I fixed with 4% paraformaldehyde, then added it to the well...

20 July 2024 3,797 2 View

How effective has the United Nations been in addressing the conflict and its consequences?

After World War, we have seen the formation of the United Nations. The sovereign body for peacekeeping but with regards to the Russia-Ukraine war it is visible that the UN seems to be ineffective...

18 July 2024 4,674 4 View

How to activate 4E8 T cells with plate-bound anti-CD3 for a genome-wide CRISPR screen?

It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...

17 July 2024 3,371 0 View

Questions of antibodies selection?

Hi there, I'd like to use immunocytochemistry to determine a surface protein expression on mouse cell. The primary antibody I have is mouse anti mouse, and the secondary antibody I have is goat...

15 July 2024 6,008 5 View