I was using Sigma Aldrich Dansyl Chloride (03641) suitable for HPLC derivatization on a method for quantification of hormones, but then I changed it for product (39220) for fluorescence and now I do not have the same results as before, are there differences on the productos?
Hello, Jose,
I'd like to contact Sigma Aldrich. At first glance, both order numbers seem to be the same compound. But the prices differ a lot. There is certainly a reason for this and it would probably also explain the different behavior during derivatization. From the key data (purity) I would have guessed that there are no differences here, but apparently there are. I would be interested in Sigma Aldrich's answer. In the laboratory one often tries to save costs, but in the special case one deals with more problems than one would like.
Regards
Markus
Hi, Jose,
Generally speaking, HPLC grade chemicals are glass distilled, submicron filtered, and undergo extensive specification testing(03641).That's why they are more expensive than BioReagent grade chemicals (39220) which are suitable for molecular biology and cell culture. The link below shows the applications of different grade solvents. https://www.sigmaaldrich.com/technical-service-home/product-portfolio.html
Good luck
Mansour
This is the answer I got from Sigma Aldrich:
A comparison of 03641 and 39220 shows that they are the same chemical. The quality testing that is used to release the two products is almost identical. Thus, we can find no indication of why the second product would not work as well as the first product. We have received no quality complaints from customers for 39220 since 2014. "
I reordered the one that was working correctly I will update once I have new results. Thank you Mansour and Markus for your help ancomments.
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