Some (but not all) DNA polymerases such as Klenow fragment, Taq, and Phi 29 DNA polymerase can catalyze strand displacement synthesis. This is evidenced by opening of molecular beacons and Loop Mediated Isothermal amplification (LAMP).
in strand displacement synthesis, the primer strand is extended using the template strand and each nucleotide incorporated displaces the 3rd strand that was originally annealed to the template?
where is the third DNA strand? the one being displaced? crystal structures and cryo-EM structures of linear primer template dsDNA structures with magnesium and dNTPs and DNA polymerases are not informative about where 3rd strand of DNA is. The displaced strand is not the template and is not the primer.
Where is the displaced 3rd strand of DNA?
Yes, that’s correct! In strand displacement synthesis, the primer strand is extended using the template strand, and each nucleotide incorporated displaces the third strand that was annealed initially to the template.
The third strand is a short, single-stranded DNA annealed to the template strand and acts as a stabilizing agent for the displaced strand . The displaced strand is the second strand of the double-stranded DNA being synthesized.
More at:
- Mechanism of strand displacement DNA synthesis by the coordinated activities of human mitochondrial DNA polymerase and SSB | Nucleic Acids Research | Oxford Academic (oup.com)
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