as a way of inhibiting pgp activity by targetting the ATPase
You could probably use a non-hydrolyzable ATP analog, such as AMP-PNP, AMP-PCP, or ATP-gamma-S. The required concentration will undoubtedly be quite high (mM).
thank you Adam B Shapiro Do you have an idea how those analogs inhibit as in the mechanism?
They compete with ATP, but are not hydrolyzed to ADP.
I am wondering if anyone has studied the relationship between human rights impact assessments and/or free, prior and informed consent processes and company-community conflict. In other words, has...
31 August 2023 890 1 View
(e.g. athletes and geniuses)
21 February 2023 6,420 1 View
i seek to know what mechanisms are involved in the upregulation or overexpression of the pgp efflux pump in the presence of a substrate
07 August 2019 8,629 6 View
looking more recent work, either effects to the development in the egg or viability after exposure or growth after exposure
12 February 2015 3,009 2 View
this is related to Eu doped vanadate based nanophosphor
06 July 2024 2,865 2 View
I am trouble shooting a malachite green ATPase assay using a kit from Sigma (MAK307) for a cytosolic protein that converts ATP --> ADP + Pi and am wondering what cheap + commercially available...
30 March 2024 9,659 1 View
I am trying to establish a PK/LDH system to detect ATP turnover. We are having difficulty observing any measurable change in NADH fluorescence (we are not using the absorbance approach), and...
16 January 2024 2,680 1 View
Does anyone has the profile of E coli. ATPase complex (F1Fo) size-exclusion chromatography (using a Superose 6 column), I mean what the profile looks like, how many peaks? Are they all well separated?
28 December 2023 1,278 0 View
Hello, During skeletal muscle contraction and during the actin–myosin ATPase cycle there is release of Pi and ADP. Can anyone tell me and ideally suggest a reading list of where they actually...
14 November 2023 6,583 4 View
Hello. Recently, I want to inject the adenosine in vivo to see its effect on the behaviors. But I am not sure whether it can cross blood-brain barrier. If it could, I would choose easier...
27 October 2023 5,687 2 View
What does it mean that it could have precipitated? What does it mean that NADH solution can be unstable? In some instances the absorbance increased from before to after I mixed in the...
12 April 2023 9,978 0 View
I'm doing ATPase activity assays, and trying to minimize free phosphates in the environment. I want to be able to deep clean a set of micropipettes and remove the phosphates from them. Does anyone...
10 November 2022 1,653 5 View
I am trying to figure out the best method to prepare vesicles of MDR1 (Pgp) for an ATPase assay and do not know the best method to use for preparing these vesicles. They have been growing in...
23 March 2022 8,109 1 View
For my project I have to pretreat RAW 264.7 macrophages with adenosine or 2-chloroadenosine for 1h and 24h, then followed with LPS (1ug/mL) treatment for another 24h. However, I'm not sure whether...
15 March 2022 4,004 2 View