Are there any other compounds known to inhibit the ABCB1 ATPase apart from vanadate?

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

What is the mechanical advantage of the K+-circulating system of cochlea? I thought simple ATPase structure would be enough to maintain [K+]. ?

Cochlea has a highly sophisticated system to maintain K+ concentration in the endolymph and IHC. I thought adopting Na+/K+ ATPase(just exchanging K+ mutually, directly) would be a lot simpler to...

14 December 2020 9,240 1 View

a Ferrari and an Oldsmobile with the same function?

I apologize for the analogy if you are really interested in cars. This is about protein function, but a crucial one because the extraction from membranes of a mistargeted protein is an essential...

15 October 2020 6,147 3 View

ADP-Glo test for ATPase activity of NLRP3?

Hello colleagues! I want to investigate the atphase activity of nlrp3 in the ADP-glo test. but I don't know what type of buffer to use for analysis. Can anyone help me?

12 September 2020 7,596 3 View

Is ATP the best energy providing molecule? can there be something better?

ATP energy-storing molecule as is no doubt an efficient! as not only it is used as energy storage and provider in all forms of life and has probably been around for billions of years. Even in the...

08 February 2020 9,015 6 View

What is the protocol that I can follow to join two RNA aptamers?

Currently I have two RNA aptamers, I read couple of papers that I can link 2 aptamers by poly adenosine linker but I do not know how to do that. If you have other suggestions of how to link two...

09 October 2019 9,327 2 View

Could someone recommend good antibodies for detecting M1, M3 AchR and SERCA1/2/3 in WB and IHC?

I'm working in a project related to Ca2+ signalling and I've to detect protein expression of M1/M3 Acetylcholine Receptors and SERCA1/2/3. Could someone recommend good antibodies for detecting...

08 September 2019 4,123 2 View

Can one compare the total energy of diprotonated and triprotonated states?

I have been studying the protonation states of an enzyme. It has a vanadate cofactor. The vanadate can be in mono-, di-, tri- or quad state. I can calculate the total QM/MM energy of say the...

07 August 2019 3,253 3 View

Can anyone recommend a Luminometer for ATPase assays?

We are currently in the market for a Luminometer tom perform the ATPase assay more efficiently. Does anyone have any recommendations with regards to model?

04 May 2019 4,132 2 View

Does anyone have a working metachromatic ATPase staining protocol in mouse skeletal muscle tissue?

I have tried the Ogilvie protocol with prestain buffer at pH 4.3 and 4.5 but the fibers are either all light blue or dark blue respectively. I've come across many papers which claim to use this...

03 April 2019 3,200 4 View

Adam B Shapiro

You could probably use a non-hydrolyzable ATP analog, such as AMP-PNP, AMP-PCP, or ATP-gamma-S. The required concentration will undoubtedly be quite high (mM).

Moira amanda Mubani

thank you Adam B Shapiro Do you have an idea how those analogs inhibit as in the mechanism?

They compete with ATP, but are not hydrolyzed to ADP.