Dear Furkanur

below a good information about your question:

Donlan, Rodney M. 2002. Biofilms: Microbial Life on Surfaces. Emerging Infectious Diseases. Vol. 8, No. 9: pg. 881-890.

No, some bacteria use preferentially the swim, lose their capacity at to form the biofilm, but it is a problem because they are less resistant at environmental stress and antibiotic

Bacterial mobility is only one aspect that contributes to biofilm formation. Many nonmotile species make good biofilms using non flagella mediated adherence mechanisms. It is naive to say all bacteria make biofilms, in biology and bacteriology especially, there is overwhelming diversity and it is likely some bacteria species have adapted to situations not requiring biofilm formation for optimal survival. Often aggressively pathogenic bacteria are less likely to form biofilms in a host. A laboratory biofilm adherence screen of MRSA blood isolates will result in a complete range of biofilm forming capacity for instance. 

Thanks all for your opinion..... it really helps me to get a nice concept

I guess it is safe to say that all bacteria can form a biofilm. Whether they choose to form it or not is a more complicated issue...

Bacterial biofilms in nature are comprised of consortium of bacteria. So it might be possible that as a single species it doesn't form biofilms, but can exist in multispecies biofilm. 

Hi. Though I would like to agree with Mr Filipe's reply, there are many reports of non-biofilm forming strains that are used as controls in biofilm formation and even gene work in biofilm related researches (controlled environments), such as Staphylococcus epidermidis (ATCC 12228). So, is it possible that though conditions permit and the microbe would persist if they did form biofilms, they simply are unable to? perhaps due to their genetic make-up? - for e.g genes involved in producing the required proteins to aid adhesion, matrix production and maintenance are not present/regulated?

Yes, even i am looking for commercially available strains that do not form biofilms to be used as control. ATCC only has Staph. Could anyone share any other source?

Thanks!

okay then.. what does it mean by non-biofilm producing reference strain ATCC 27853?

http://www.scielo.br/pdf/bjid/v15n4/v15n4a01.pdf

Hi Furkanur. If not only motility assisted biofilm formation, there are various other genes, quorum sensing molecules and proteins that cause or enable the bacteria to form biofilms. So it would be sensible to say that the lack or absence of these 'triggers' may cause the bacteria to not form or form very weak (and perhaps eradicable) biofilms. 

Hi Vandana, I did come across the same issue when selecting control strains for experiments, so I get your point of approach. In my experience with S. epidermidis, when they mention non-biofilm forming/biofilm negative strains, it is not that these strains do not form adhering structures at all (which I expected to see as well), but rather form very thin or weak biofilms compared to the biofilm forming strain (that clinically would relate to strains not posing serious harm to humans as they may be eradicable). This was seen when I conducted the conventional microtitre plate method to cultivate biofilms and crystal violet staining of both non- and biofilm forming strains of S. epidermidis.  

Hi Tripti. You may want to read on the strains you plan to use. S. epidermidis has some strongly biofilm forming strains. They mostly cause invasive medical device related nosocomial infections (apart from other biofilm related infections). Good Luck. RK

Probably Pelagibacter and other planktonic bacteria in the sea never develop biofilm in their life cycle.

that bacteria recommend to use as a control in a biofilm formation assay of Listeria monocytogenes? Ramona Khanum Md. Furkanur Rahaman Mizan

Hi Nicolas Melo... I work with control for Staph epidermidis. I'm not aware of those used for L. monocytogenes biofilms at the moment. Sorry.