Ramona Khanum

3 Questions 35 Answers 0 Followers

Questions related from Ramona Khanum

What are the possibilities of culturing from the MIC and finding no colony growth, (making it also an MBC)?

A concentration fold higher and lower than that of the MIC were culture spread onto agar mediums, no growth was observed for the MIC value and a concentration higher. This makes the MIC also an...

20 July 2013 10,047 9 View

Steps in crystal violet staining cause biofilm to dislodge. Can I compare OD of negative control and formed biofilm without staining?

Biofilms formed by biofilm forming ATCC strains in 96- non culture & culture treated well plates and fixed with methanol tend to partially dislodge during washing and dye dissolution steps. One...

15 April 2013 5,514 3 View

I have been trying to develop a HPLC method for Meropenem trihydrate but failed in many attempts. Are there any suggestions?

I have tried the following mobile phase solvents and ratios of them. I am using 1ml/min FR and 298nm WL. Either I do not get the peak, it is too near the solvent peak, peaks not reproducible or...

05 October 2012 6,106 35 View