Are there any methods other than adsorption to remove non specific activity of serum in order to perform ADCC assays ?

11 May 2018 0 2K Report

Multiple adsorption cycles of antibody(obtained after immunizing rabbit with human cell derived antigen) with human cells has been working till n n till now to remove non-specific activity of serum( human cell derived activity). However with serum obtained from monkey , the adsoption cycles doesnt seem to work. Do you have any possible explanation for this ? any alternate approach ?

Badges
Science topic

Similar topics
Microbiology
Assays

More Bishal Marasini's questions See All

What are the implications of Biochar, Carbon farming, Conservation ecology, and fair consumption towards Sustainability?

How does the above items impact the goal towards net zero emissions? What might be the role of the emerging scientists, leaders and professionals for achieving sustainability in agriculture...

26 June 2024 5,678 3 View

Is there any rules that we can cite our literature review from the podcasts or videos from official sites where the writer had spoken?

Can we cite our literature from videos or podcasts from official websites?

21 June 2024 5,706 1 View

What is the best and fast way to complete literature review fast?

How to complete literature review fast without using AI tools and with no plagiarism? Why it's hard to get citations? How can we get articles that we can cite fast?

17 June 2024 677 2 View

What is the best possible way to evaluate the amount of Soil organic Carbon content (SOC) in the soil?

Is Walkley and Black method is the only method that is universally accepted to measure SOC evaluation? Are there any other methods that determines the SOC content with minimal errors?

17 June 2024 1,725 0 View

The large odds ratio and the wide confidence interval, what are the potential reasons for these findings? Is this result reliable?

In my study, I found an odds ratio (OR) of 65 with a 95% confidence interval (CI) ranging from 1.2 to 195. Given the large odds ratio and the wide confidence interval, what are the potential...

07 June 2024 402 3 View

What are the effect on nodulation in mungbean by using selective herbicides? What herbicide might be best to minimize impact on root nodulation?

hand weeding is time consuming, costly and other mechanical weeding practices are not available here. I am seeking for effect of selective herbicides on root nodulation in mung bean farming....

22 May 2024 4,141 0 View

What are the essential data and calculations to be taken for the research that aims to increase soil Organic matter using mungbean as cover crop?

I am a researcher of Soil science department. 5 months ago I had planted wheat for my research. The research design was split plot design. it has 3 replications, each replication has 2 main plot...

05 May 2024 4,815 3 View

If the seeds didn't germinate well equally on every plot and i need data from 1m2 area only. can i sow seeds in 1m2 in gap filling for research?

I am a researcher of Soil science department. 5 months ago I had planted wheat for my research. The research design was split plot design. it has 3 replications, each replication has 2 main plot...

28 April 2024 7,432 5 View

How often do I have to irrigate the field for germination of mungbean where flooding is not allowed during very hot summer days?

Day temp. Above 40 degrees, low RH below 13%, high wind speed of 50km/hr, in clayey soil, over irrigation causes crusting which affects germination, thus flooding can't be done.

23 April 2024 4,821 0 View

Do Mungbean germinate on tropical region where max. day temperature is 43 degree celcius, Relative Humidity as low as 13%, & wind speed of 33km/hr?

Pre sowing irrigation was applied, irrigation after 2 days of sowing in clayey soils of terai region of Nepal 🇳🇵,very low RH, hugh wind speed and day temperature avove 40 degree celcius and heat...

23 April 2024 3,943 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Absorption coefficient of methane?

Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?

06 August 2024 980 5 View