02 February 2013 4 2K Report

I want to estimate plant total genome size by flow cytometry. Are there any reliable, simple protocols to estimate 2C content?

More Biswajit Bose's questions See All
How to calculate theta, theta prime, coalescent depth and minimum interspecific distance?

I am working on DNA barcoding of two closely related plant species. I have used 5 marker regions for my analysis. How to calculate theta, theta prime, coalescent depth and minimum interspecific...

10 November 2016 9,894 3 View

Input matrix for Splitstree for Dominant markers using binary data ?

I am working on conservation genetics of some alpine medicinal plants. I have used some dominant markers and co-dominant markers. I am unable to construct network tree by using Splitstree. Can...

04 May 2016 1,112 4 View

What are "Unverified" sequence in Genebank? Can I take those unverified sequences from Genebank for my phylogenetic analysis?

I am working on molecular phylogeny in the order level. When I am extracting the sequences from NCBI Genebank, I found there are lot sequences marked as unverified. For concratenation of multiple...

04 May 2016 6,523 7 View

How much impact factor do we need to get a PDF in plant science in European country or in USA??

Impact factors are playing a pivotal role now a days in the age of competition. Good work or high impacts....??? How many modern techniques or simple yet novel breakthrough ....???? New idea or...

01 February 2015 8,639 2 View

How to deal with the problem in Internal transcribed spacer 2 amplification?

I am working on some plants from Valerianaceae family(angiosperms). I have already sequenced rbcl, matK and two inter-genic spacer regions. I want to amplify ITS1 and ITS 2 regions. Here,...

08 September 2014 3,923 8 View

Can you help me with the operation of Mr Bayes software and the procedure for Nexus file conversion?

I want to use Mr Bayes for Bayesian inference model choice across a wide range of phylogenetic and evolutionary models (MCMC analysis). Which software will be best to convert the alignment files...

06 July 2014 9,361 4 View

What are the steps before sequencing of a PCR purified product for rbcL, matK, psbA-trnH, trnL-F?

I have already amplified rbcl, matk, trnl-f, psba-trnh and ITS regions of my study plants. Now I will be doing sequencing of those regions. I have purified those PCR products by Quaquick gel...

06 July 2014 8,974 5 View

Can anybody help me with ITS 2 and ITS 1 amplification?

I got very nice bands with ITS 2 and ITS 1 in my study plant samples. I collected them from remote areas of India. Recently, in both ITS markers, I am not getting the bands. I have checked my...

05 June 2014 4,071 13 View

How can I generate a contig from raw sequence data?

If one gene is over 700 bp long then how to get the consensus sequence data from the raw sequence data? I have done the sequencing using both forward and reverse primers. Also, I want to know the...

05 June 2014 9,465 6 View

I got a peak at 422/423 nm in UV spectra. What is the size of my Ag nanoparticle?

Does colour and size of silver nanoparticles depend on each other? I used to get a peak at 430-450nm in UV spectra, but now I am getting a peak at 423nm. Sometimes I get double peaks (one small @...

05 June 2014 9,090 3 View

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