How to calculate theta, theta prime, coalescent depth and minimum interspecific distance?

I am working on DNA barcoding of two closely related plant species. I have used 5 marker regions for my analysis. How to calculate theta, theta prime, coalescent depth and minimum interspecific...

10 November 2016 9,669 3 View

Input matrix for Splitstree for Dominant markers using binary data ?

I am working on conservation genetics of some alpine medicinal plants. I have used some dominant markers and co-dominant markers. I am unable to construct network tree by using Splitstree. Can...

04 May 2016 928 4 View

What are "Unverified" sequence in Genebank? Can I take those unverified sequences from Genebank for my phylogenetic analysis?

I am working on molecular phylogeny in the order level. When I am extracting the sequences from NCBI Genebank, I found there are lot sequences marked as unverified. For concratenation of multiple...

04 May 2016 6,268 7 View

How much impact factor do we need to get a PDF in plant science in European country or in USA??

Impact factors are playing a pivotal role now a days in the age of competition. Good work or high impacts....??? How many modern techniques or simple yet novel breakthrough ....???? New idea or...

01 February 2015 8,537 2 View

How to deal with the problem in Internal transcribed spacer 2 amplification?

I am working on some plants from Valerianaceae family(angiosperms). I have already sequenced rbcl, matK and two inter-genic spacer regions. I want to amplify ITS1 and ITS 2 regions. Here,...

08 September 2014 3,814 8 View

Can you help me with the operation of Mr Bayes software and the procedure for Nexus file conversion?

06 July 2014 9,232 4 View

What are the steps before sequencing of a PCR purified product for rbcL, matK, psbA-trnH, trnL-F?

I have already amplified rbcl, matk, trnl-f, psba-trnh and ITS regions of my study plants. Now I will be doing sequencing of those regions. I have purified those PCR products by Quaquick gel...

06 July 2014 8,826 5 View

Can anybody help me with ITS 2 and ITS 1 amplification?

I got very nice bands with ITS 2 and ITS 1 in my study plant samples. I collected them from remote areas of India. Recently, in both ITS markers, I am not getting the bands. I have checked my...

05 June 2014 3,943 13 View

How can I generate a contig from raw sequence data?

If one gene is over 700 bp long then how to get the consensus sequence data from the raw sequence data? I have done the sequencing using both forward and reverse primers. Also, I want to know the...

05 June 2014 9,342 6 View

I got a peak at 422/423 nm in UV spectra. What is the size of my Ag nanoparticle?

Does colour and size of silver nanoparticles depend on each other? I used to get a peak at 430-450nm in UV spectra, but now I am getting a peak at 423nm. Sometimes I get double peaks (one small @...

05 June 2014 8,892 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View