Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How to combine three-part variable into one variable?

I asked the same respondents about how much luck, their own efforts and connections they need to achieve Goal # 1, # 2, # 3, # 4. (first group). The second group had the same questions and the...

09 August 2020 3,502 5 View

Cross-cultural adaptation of a questionnaire: what are the requirements for a translator?

27 February 2020 2,289 11 View

No title

23 February 2020 5,145 7 View

After adding stop solution (sulphuric acid) in reaction mixture of HRP assay with TMB, yellow color becomes brighter. What is the mechanism?

Yellow colour, before the reaction is stopped, is due to the formation of oxidized TMB, which isTMB(2+). The reaction is stopped by degrading HRP, but are there any further oxidations happening?...

13 December 2019 269 3 View

What are advantages and disadvantages of inducible promotors, namely of positive and negative regulation?

When we have a positive regulation the control is tight, so we have low background expression under non-induced conditions. Negative regulation can not be fully controlled, but as far as I...

25 January 2019 2,007 5 View

Has anyone had a problem with HEK293 insensitivity to Carbachol?

Hi everybody! Would be very thankful if someone can help with a small problem! Suddenly HEK293 became insensitive to Carbachol stimulation in FURA experiments. With application of Carbachol no...

23 November 2015 1,873 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials?

Hi, all! How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials? I have not find the choice for explicit element in Abaqus CAE. I am wondering whether it is possible...

25 February 2021 9,936 2 View

Any recommendations for methods to analyze elephant space-use (using GPS) and correlating this with changes in NDVI?

For my Masters I am looking into elephant space-use and its effect on vegetation change. I have elephant GPS collar data and was going to use Google Earth Engine to obtain NDVI values. I would...

16 February 2021 7,097 3 View

How to consider effects of rapid warm adaptation in modeling of ecological impacts of warming?

I would highly appreciate it if my fellow ecologists (biologists) provide their opinion on the thoughts below [esp., shortly tell us which path may be more effective, if they know another way, if...

15 February 2021 3,642 6 View

[Sand Lizards tracked with VHF telemetry] How do I analyze the results of my home range estimations properly?

Hello, I just recently started working with the different types of home range estimations and have a few questions about this and that. Maybe you could help me out with that?! My study animals are...

03 February 2021 5,573 3 View

What is the magnitude of yearly rhizopdeposition in tropical forests?

Hi all, I am trying to find reference values on annual soil rhizodeposition rates for different kinds of forests, with a particular interest for tropical rainforests. Do we have an idea of the...

26 January 2021 4,278 4 View

Can you recommend a good book on analytical chemistry for non-chemists?

Dear colleagues, I am asking for you recommendation: I need a book on analytical chemistry in environmental sciences. As a non-chemist (a biologist) I have some basic chemistry knowledge but not...

25 January 2021 8,486 8 View

Why are my Maxent SDM projections coming up almost entirely blue?

Hello Maxent Community, I have been generating SDM models and projections in Maxent. I have had some success with one set of variables (some Hydroshed variables mixed with World CLIM variables);...

19 January 2021 3,994 7 View

In which case meta-heuristics algorithms cannot minimize the network error?

Hello, I am working on hybrid neural network. I have combined ANN with two different evolutionnary algorithms to build a prediction model of 6 inputs and one output with one hidden layer of 7...

18 January 2021 7,144 3 View