Are there any differences between raw and frozen-thawed bull sperm prior to flow cytometric analysis?

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Multigroup analysis by gender?

Research model have three independent variable and one dependent variable. Basically i want to check the entrepreneurial intentions of business students. Nature of study is quantitative and i'll...

18 October 2020 1,176 3 View

Forcast of any Index is possible or not?

to forcast some indicator, can we directly forcast Index variable or we have to forcast all the poxies, used to calculate that index to forcast that index. for example to forcast inflation, can we...

29 November 2019 5,028 3 View

How to find rhamnose in plant (wheat) under stress and seed priming?

01 September 2019 5,478 2 View

To identify structural break, which method is appropriate?

to capture structural break in panel data series, whether we must add time dummy in regression equation or must apply chow test? which method would be better?

16 May 2019 157 3 View

How to perform a REFA (Regularized Exploratory Factor Analysis)?

Hello everyone! I am performing a personality study to unravel the personality dimensions in an African primate species. The group of subjects accounts for five individuals what makes my sample...

06 July 2014 839 5 View

How to perform a REFA (Regularized Exploratory Factor Analysis)?

Hello everyone! I am performing a personality study to unravel the personality dimensions in an African primate species. The group of subjects accounts for five individuals what makes my sample...

04 July 2014 4,101 4 View

Has anyone used DEA (Data Envelopment Analysis) in research work?

Could anyone suggest some useful and easy to understand references?

19 February 2014 5,487 3 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View