Are there any differences between raw and frozen-thawed bull sperm prior to flow cytometric analysis?

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What is the best storage method for eluted samples for Infectious Diseases panels for Molecular PCR testing?

We run Fungal Panel using Molecular PCR Technique. We extract Nuclease Free water in the same way as we extract the sample. This eluted NFW is used as negative control on our PCR Plate for quality...

16 April 2024 776 1 View

How to do calculation for CVI and CVR in content validation based on likert scale?

In order to calculate CVI and CVR, the 4-points of Likert Scale frequently being adapted to determine the item validation. The indicators of 4-points used degree of relevance (1-not relevent,...

04 March 2024 2,838 2 View

Are there any recommended references in selecting experts before conducting pilot testing of questionnaires in service quality of public transport?

I am looking for references to look for the criteria of experts groups before conducting pilot testing. The experts i believe will receive the questionnaires to review before the real study being...

25 February 2024 1,519 3 View

Can anyone suggest why the U87-MG glioblastoma cell line did not attach after thawing?

The cells do not confluency after thawing. Even after 10 days, the color of the medium does not change. I change the medium, the non-adherent cells disappear and then the density in the flask...

11 December 2023 7,002 2 View

What is the most accepted guideline for Waste management plan for veterinary hospitals/ veterinary laboratories.?

what is the most (internationly) accepted waste management guideline for veterinary hospitals and veterinary laboratories for medical waste in veterinary hospitals and laboratories, how can we...

24 July 2023 2,006 3 View

What the reason PCR identification E.coli using usp A gene?

What makes usp A (universal stress protein) gene considered valid for molecular identification of Escherichia coli bacteria (used as PCR Primer)? Why use usp A while there are several groups of...

18 June 2023 4,022 2 View

How to dissolve plant ethanolic extract for seed germination assay?

How to dissolve plant ethanolic extract in sterile aquades if it is not dissolving completely? Should i dissolve it first using DMSO or ethanol in low conc before dilute it with aquades?

29 March 2023 2,769 1 View

Can someone suggest a good advanced reference Book of Public Finance for Economics?

Hi: I need any advanced Public Finance Reference Book for post-graduate studies. the reference with mathematical applications or methods. Regards

03 December 2022 4,396 5 View

We need to do a multivariate regression with two dependent variables and three independent variables. Can anybody suggest me which software to use?

We need to understand the relationship between two dependent variables (Frictional Noise and Coefficient of Friction) and three independent variables (Material Hardness, Surface Roughness, and...

12 July 2022 257 2 View

How to create an Ensemble of model outputs?

I have raster images from three models and I want to create their ensemble output. The correlation among rasters is near 70%. Is it a scientifically true approach? Can we combine 3 model outputs...

05 July 2022 5,345 3 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,484 5 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

Problem with Two-phase simulation?

Hello; I am conducting a CFD study on two-phase flow in a separator, utilizing refrigerant as the working fluid. For this study, I have implemented pressure inlet and pressure outlet boundary...

25 July 2024 2,309 5 View