I found some data, but there are all indirectly
Hi, I'm transducing Ly1 and L363 cell lines using our standard protocol for retroviral transduction. The cells are successfully transduced as evidenced by GFP expression. However, after 4-5 days...
26 January 2021 4,071 3 View
I need to find metals, plastics or metal alloys that have a specific Vickers scale hardness scores. I'm not aware of any existing web sites and tools that could aid me in searching for this data,...
06 August 2020 4,842 4 View
Hello, We're conducting quantitative research as a part of this semester, and the analysis method of choice is multivariate linear regression. Right now, we're checking for the fulfillment of...
03 November 2019 2,694 3 View
In general, sex ratio is expressed as the ratio of males to females in a population, ie number of males/number of females (*100). However, when the number of females in (sub)population is 0...
18 August 2019 1,856 28 View
Hi, I'm currently having problems with Western blots using the following antibodies from Cell Signaling: Phospho-Stat3 (Tyr705) STAT3 (total) Phospho-Stat5 (Tyr694) STAT5 (total) They seem to...
11 August 2019 3,463 4 View
Since using PLE carriers can be excited to the bottom of the conduction band, where they kinetic energy is zero it should be less probable to detect luminescence coming from carriers influenced by...
10 July 2019 3,335 3 View
Can I transfect HEK293 cells using siRNA purchased form Dharmacon and use Lipofectamine reagent instead of DharmaFECT? I have been using electroporation for the transfection, but it seems like HEK...
21 January 2019 2,884 3 View
Hi, I want to do an overlap extension PCR (OE-PCR) using a PCR-amplified plasmid backbone and a PCR-amplified insert. The OE-PCR shall add the insert into the plasmid similar to this...
12 July 2018 1,976 3 View
I am from Czechia (in past Czechoslovakia). I do research to check if the problems in choosing a field of study (measurred by CDDQ) are related to MBTI typology dimensions. I know this method is...
28 April 2018 9,264 1 View
We are looking into liquid handling systems for pipetting qPCR plates (384 well) and for NGS library preps (amplicon seq). There are many machines on the market that are able to do that (Biomek...
25 February 2018 8,701 5 View
Anyone can recommend any books on such topics? Thanks in advance.
02 March 2021 8,457 2 View
Is there a powerful system for the security of the systems distributed on IoT systems?
02 March 2021 3,858 10 View
Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....
02 March 2021 285 2 View
I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.
02 March 2021 1,516 4 View
The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...
01 March 2021 2,272 4 View
Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...
01 March 2021 9,536 3 View
As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...
01 March 2021 3,199 3 View
Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...
01 March 2021 7,168 9 View
Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?
01 March 2021 3,422 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
