I have been looking online for all in one expression vectors of CRISPR-Cas9 in plants and can't seem to find any. If anyone knows of any that are optimized for Agrobacterium transformation and can steer me in the right direction I would be grateful.
You can always derive your own from pCAMBIA backbone as described by Xie K, Yang Y. Mol Plant. 2013 Nov;6(6):1975-83. doi: 10.1093/mp/sst119. Epub 2013 Aug 17. 10.1093/mp/sst119.