Are they any Biobutadiene generating bacteria ?

Are there any naturally, occurring Heterotrophic or autotrophic bacteria, which can generate butadiene from there metabolic pathway.?

08 September 2016 8,532 1 View

HPLC or GC?

I want to detect the alcohols in my sample. So HPLC and GC (FID and TCD -Detector) which I am about to use consists of C18 column, and UV detector (For HPLC). The GC is equipped with agilent...

31 December 2013 2,356 13 View

What would be the cost for a single waste water sample?

I wanted to begin a molecular biology analysis, so, I e-mailed the macrogen (Korean Branch) for 454 pyrosequencing cost. They replied to me as below, could someone kindly explain their...

11 December 2013 2,422 1 View

Do you have references regarding the use of ceramic membranes in microbial fuel cell?

Can anyone give me a few references showing the internal resistances of ceramic membranes in microbial fuel cell?

11 December 2013 609 5 View

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02 March 2021 324 2 View

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02 March 2021 3,060 3 View

Do anyone here can help us in the field of molecular docking?

We have synthesized various metal complexes with different environment around the metals. Now to see the application of synthesized complexes we need this study also. we are also interested in...

01 March 2021 1,899 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View