I am having a bit of trouble growing the myometrial cell line of mice if any one can share the protocol or some paper?
Feeding zebrafish larvae properly is a challenge if we need to grow them. According to standard guidelines, how many grams or milligrams of Artemia should be given to 6 dpf larvae?
13 June 2024 389 0 View
Can anybody recommend the tensile strength of cement mortar? Can ASTM C307 be used to test for mortar without fibers?
03 June 2024 7,550 1 View
for ABO3 if only A & B then we used t=rA+Ro/sqrt2(rB+rO) kindly explain for BaSrCaTiO3 ?
31 May 2024 2,036 0 View
some data shows direct and some inverse relation? kindly share any authentic paper please.
07 May 2024 6,657 4 View
I need to prepare silymarin cream , I am facing some difficulty with that
06 May 2024 3,614 0 View
Kindlly provide links or literature about the mechanism of free radical copolymerization of styrene and methacrlate, kinetics and rate equation
27 April 2024 404 2 View
I and using vectors with autoinducibe promoters(Pylb and cyt1). I have performed SDS of 24, 48, 72, 96 and 120 hours. No expression is found in soluble or insoluble form.
14 March 2024 8,752 0 View
I induced the preadipocyte after they reached 100% confluency. Then, I treated them with differentiation medium (hg DMEM 10% FBS, 1X P/S, IBMX 0.5mM, Dex 1uM, and Insulin 10 ug/ml) for 2 days....
18 February 2024 8,219 0 View
Is there any way to add multiple motion files in a single go to a deepsoil software or to run Shake91 in loop form
30 October 2023 8,858 0 View
The bright zone is high in REEs up to 4% and other zones are depleted in REEs. Can we say overgrowth? But they are enclosed in apatite middle.
25 September 2023 4,667 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View