Why doesn't my RNA loading look uniform?

I am running formaldehyde-agarose gel electrophoresis to perform non-radioactive northern blotting. I know northern blotting is a very old story now, however, I am trying to study different...

21 September 2020 8,153 4 View

How to design "A Tunable RF Front-End With Narrowband Antennas for Mobile Devices" ?

I don't know how to design the architecture using the PCB board and how to test the fabricated prototype ? can you help me out of this issue?

17 December 2019 9,068 4 View

Between Julia and MATLAB, which would be the most efficient for a discontinuous Galerkin FEM code?

I need to simulate a 2-D problem with DGFEM. I have been advised to use C/C++ for a code that delivers quick and efficient results, however, I am not very comfortable with them, and my base code...

16 December 2019 7,001 5 View

Can total energy Vs k-mesh increase to higher values in DFT-VASP?

I am performing DFT calculations on halide perovskites using VASP code. I began my calculations on CsPbI3 where I took 6.2 Angstrom as lattice parameter and built the cubic structure in VESTA....

16 September 2018 9,316 6 View

Can you please suggest a good bioinformatics textbook/guide for gaining expertise on multiple sequence alignments?

Hello all, I am looking for recommendations on a good bioinformatics textbook/guide which helps me gain sound background knowledge on multiple sequence alignments. A part of my research is to...

31 October 2017 1,582 3 View

96 well plate procedure for TPC and TFC?

31 January 2017 2,927 4 View

How can I minimize the air bubbles during a western blot wet transfer?

Hello everyone, I am right now facing the issue of air bubbles during the western blot wet transfer method. It is so frustrating that some silly air bubbles mess up the whole results. This is what...

20 March 2015 6,082 27 View

How can I induce DNA damage using Temozolomide?

Hi all, I am currently studying activation of PARP after inducing DNA damage. The method I follow is: 1) exposing tumor cells (HCT116, HeLa cells) and MEFs (WT and S12) to DNA damaging agent...

01 March 2015 3,311 3 View

Can anyone explain to me about tagged western blotting?

I am curious about the tagged western blotting, where, proteins are probed on a nitrocellulose/PVDF membrane with HIS-tag/GST-tag primary antibody initially, and later with a corresponding...

01 March 2015 6,492 3 View

What is the BRCA status of the colon cancer cell line HCT116?

I am trying to put together the BRCA and p53 status of various cell lines like HeLa, WT/S12 MEFs, HCC1937, MDAM231, MCF7, HCT116. I was able to get the statuses of every other cell line, but just...

30 November 2014 2,838 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Physics: Why do we describe light by referring to its wavelength, but microwaves and radar pulses by referring to their frequency?

In physics, the description of the properties of light generally refers to its wavelength (nm), while in the range of microwaves and radio waves we refer to the frequency of a signal (e.g. kHz or...

03 March 2021 1,682 3 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View