We know that many genes are expressed in a tissue-specific manner. Mechanisms include the remodeling of heterochomatin and euchromatin. I wonder now if there's any way of high-resolution chromosome banding could show such difference? Or is there any other ways of staining could indicate that one gene is located in heterochromatin or euchromatin?
Thanks!
ChIP-Seq. For example,
Euchromatin = H3K4me3/H3K36me3
Faculatative heterochromatin = H3K27me3
Constitutive heterochromatin = H3K9me3/H4K20me3.
See this for example http://www.nature.com/nature/journal/v448/n7153/full/nature06008.html
Generally not even the resolution of high-resolution chromosome banding will be sufficient to show such differences...
However, polytene chromosomes may be an exception: http://www.nature.com/cr/journal/v3/n1/full/cr19931a.html and atomic force microscopy may provide an option http://www.ncbi.nlm.nih.gov/pubmed/22633852
Hi,
Chromosome banding patterns show gross differences...they may or may not show fine tissue specific expression differences.....You may be able to use FISH analysis along with banding patterns to localize a particular gene in a hetero- or euchromatic region..
Hi Ajay, thanks for your answer. Actually I'm quite a bit confused by the identification of hetero-/euchromatin. I read that the way to identify hetero-/euchromatic region on chromosome is the C-banding, however, C-banding is also of low resolution. Do you know if there's way to identify hetero-/euchromatin in metaphase? or even in interphase?
ChIP-Seq. For example,
Euchromatin = H3K4me3/H3K36me3
Faculatative heterochromatin = H3K27me3
Constitutive heterochromatin = H3K9me3/H4K20me3.
See this for example http://www.nature.com/nature/journal/v448/n7153/full/nature06008.html
This might answer your question: http://www.nature.com/scitable/topicpage/chromosomes-14121320
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